Costs

As there are plenty of specific protocols for library preparation and Next Generation Sequencing available we do not provide a complete summary of costs calculations on the website. Therefore, this is just a brief overview for the most requested protocols. Please feel free to contact us for detailed information and current cost estimations for your sequencing project.

3’-mRNA Expression profiling

The optimal solution for identifying genes that are differentially expressed between samples.

We offer expression profiling for three types of total RNA input:

• Standard: >= 100ng High-integrity total RNA
• Low-input: 10 – 100 ng High-integrity total RNA
• FFPE: FFPE-derived (or otherwise very degraded (DV200>2%)) total RNA

Calculated costs*

Library preparation**	Sequencing	Price per sample
Standard, 6-10 samples	Ion Torrent	€370 – €260
Standard, 10-30 samples	Ion Torrent/Illumina***	€260 – €185
Standard, >30 samples	Illumina	€185 – €150
Low-input, 6-10 samples	Ion Torrent	€385 – €275
Low-input, 10-30 samples	Ion Torrent/Illumina***	€275 – €200
Low-input, >30 samples	Illumina	€200 – €165
FFPE, 6-10 samples	Ion Torrent	€380 – €270
FFPE, 10-30 samples	Ion Torrent/Illumina***	€270 – €195
FFPE, >30 samples	Illumina	€195 – €160

_{* For external users, please expect a 10% overhead}

_{** Library preparation is performed with the inclusion of internal standards (SIRVs or ERCC mixes).}

_{*** Optional, but be aware that more total reads are needed from Illumina sequencing to achieve a number of mapped reads equal to those obtained from Ion Torrent generated data.}

Sample quality criteria

To ensure optimal library construction RNA samples must be DNAse treated, dissolved in nuclease free water or low TE buffer, and have an OD260/280>1.8. The integrity of the RNA is at best when the RNA Integrity Number (RIN) exceeds 7.5.  Most importantly, for samples to be directly compared, the RINs should deviate no more than one unit between samples.

For FFPE-derived RNA, any input integrity is allowed.

The core offers help in assessing and meeting quality criteria

RNA integrity measurement	€ 10 per sample
Standard sample Clean-up	€ 10 per sample
Clean-up to remove melanin or globins	€ 10 per sample

Single-cell sequencing

We are offering:

Various types of single-cell sequencing from the ever extending 10x Genomics portfolio, including single-cell RNA-seq, Feature-seq and ATAC-seq. As stand-alone or in multi-omic combinations, and allowing you to assay from a few hundred up to 10,000 single-cells per sample. The cost vary considerably depending on project designs and applications. You should however expect the costs of sorting and library preparation to be in the range of €1700 – 2000 per sample (cell-suspension). Remember to additionally count sequencing costs, which will be determined by the number of targeted cells per sample and the chosen single-cell application.

Please contact us for more detailed information, fitting to your project!