The Next Generation Sequencing Core offers support for your Sequencing project from initial idea, selecting an optimal design, identifying the best-fit sequencing approach, and assisting in proposal writing. This applies whether you are choosing to sequence with us or prefer an ordinary of-the-shelf solution provided by commercial sequencing providers, in which case we can also help you navigate the various terminologies.
We are happy to advise on nucleotide purification methods, perform quality controls, generate libraries, and finally handle the generation of sequencing data for your project.
We have a decade of experience in adapting the tools of library generation to generate new protocols to fit special project needs. Especially for novel RNA-seq approaches (examples: mt-tRNA seq, very-low input expression profiling, total small-RNA seq (20-200nt), and expression profiling of very degraded FFPE-derived RNA) In addition to enabling successful project completions, this has led to the Core attaining designation of “ION-Torrent RNA-seq reference lab” and the winning of the international Lexogen Research Award.
We deliver filtered quality data, either in fastq or ubam format for downstream analysis. For certain applications we can additionally offer mapping to a reference genome.
As an integral part of the Medical University Biocenter, we offer several courses in NGS project design, library generation, sequencing, and data analysis.